DNA repair pathways and the effect of radiotherapy in breast cancer

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and the limited processing of your personal data in order to function. Regulation of telomere length by fatty acid elongase 3 in yeast: Involvement of inositol phosphate metabolism and Ku70/80 function Suriyan Ponnusamy, Nathan L. Alderson, Hiroko Hama, Jacek Bielawski, James C. Jiang, Rashna Bhandari, Solomon H. Snyder , S. Michal Jazwinski, Besim Ogretmen Ku70 functions in addition to nonhomologous end joining in pancreatic β-Cells. Omid Tavana, Nahum Puebla-Osorio, Jiseong Kim, Mei Sang, Stella Jang, Chengming Zhu. Ku70, to discover the effect of cellular responses to DNA damage in pancreatic β-cells on … Abstract In this study, the ku70 and ku80 homologs from the Aspergillus niger genome were identiﬁed and their function was analyzed using targeted mutagenesis. The role of the ku80 gene in non-homologous end-joining (NHEJ) was investigated by calculating the frequency of homolo-gous recombination.

In the presence of 100 μM etoposide, apoptosis was induced within 1 h in wild type DT40 cells but not in KU70-/-and DNA-PKcs-/-/-cells. In addition, the DNA-PK inhibitors NU7026 and wortmannin, as well as the caspase inhibitor Z-VAD-FMK, inhibited etoposide-induced apoptosis in wild type cells. Ku70/80 also interacts with the telomeric factor Trf1 and plays an important role in telomere maintenance (Bailey et al., 1999; Hsu et al., 1999, 2000; Gasser, 2000). Conformational changes in Ku70/80 could affect this interaction and impinge upon whether Ku70/80 binds Trf1 and acts at telomeres, or alternatively participates in NHEJ. Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818.

1979/80.'10 om kontroll av hybrid—DNA-teknikens av propositionen hemställde socialut- skottet i betänkandet SoU 1979/80:18 att Marja-Leena Ojutkangas, leg sjuksköt, forskningsassistent (80 %) Functional performance in self-care and mobility after selective dorsal rhizotomy: a 10-year practice-based Decreased Ku70 Levels in Prostate Cancer.

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Concomitantly, DNA-bound Ku70/80 proteins serve as a docking platform for sequential binding of C-NHEJ components and for an array of proteins mediating the DNA-damage response. Further, Ku70/80 have been reported to coordinate DSB repair with cell cycle arrest, pathway choice, and, if needed, apoptosis. Molecular Function; ATP binding; DNA helicase activity; damaged DNA binding; telomerase RNA binding; telomeric DNA binding; Biological Process; double-strand break repair via nonhomologous end joining; recombinational repair; telomere maintenance; Cellular Component; Ku70:Ku80 complex Ku is thought to function as a molecular scaffold to which other proteins involved in NHEJ can bind, orienting the double-strand break for ligation.

DNA DAMAGE - DiVA Portal

In addition, the DNA-PK inhibitors NU7026 and wortmannin, as well as the caspase inhibitor Z-VAD-FMK, inhibited etoposide-induced apoptosis in wild type cells. Ku70/80 also interacts with the telomeric factor Trf1 and plays an important role in telomere maintenance (Bailey et al., 1999; Hsu et al., 1999, 2000; Gasser, 2000). Conformational changes in Ku70/80 could affect this interaction and impinge upon whether Ku70/80 binds Trf1 and acts at telomeres, or alternatively participates in NHEJ. Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications.

Although human Mre11 and MR bind to the forked double strand DNA, they could not compete with Ku for DNA ends or actively mediate the displacement of Ku from the DNA end either physically or via its exonuclease or endonuclease activity. In summary, this study highlights an unexpected and important function of Ku70: In addition to repairing DNA damage in the NHEJ pathway, Ku70 also regulates pancreatic β-cell proliferation. The connection between the Ku70 level and stabilization of β-catenin merits further investigation, which could shed light on possible ways to enhance pancreatic β-cell proliferation. It is known that Ku70/80 has a high affinity for DNA ends; therefore, to exclude the possibility that the increase in 12/23 regulation was an artifact due to binding and translocation of Ku70/80 into the DNA, we performed experiments with supercoiled circular DNA. Ku70, a known nonhomologous end-joining (NHEJ) factor, also functions in tumor suppression, although this molecular mechanism remains uncharacterized.
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The Ku heterodimer provides DNA-binding and regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The heterodimer Ku70/80 plays a critical role in DNA repair and cell death induction after damage. The aberrant expression and localization of these proteins fail to control DNA repair and apoptosis. sClusterin is the Ku70 partner that sterically inhibits Bax-dependent cell death after damage in … Elo3 shares a common pathway with Ku70/80 for the regulation of telomere length. 2013-05-01 Importantly, to determine downstream mechanisms, epistasis experiments were performed, and data indicated that ELO3 and YKU70/80 share a common pathway for the regulation of telomere length.

80 a tank, so chances are very good that it will be fully functional if it looks good 20 Jul 2019 the regulation of DNA non-homologous end-joining through Ku70/80 As PAXX is a Ku interactor with a role in NHEJ, the effect of PAXX An early event at many DSBs is the binding of the. Ku70 and Ku80 (also known as XRCC6 and XRCC5, respectively) proteins, which can recruit DNA-PKcs and 25 May 2007 Summary: Subunit of the Ku70:Ku80 complex; binds RNA and damaged Composed in yeast of Yku70p and Yku80p, Ku functions in genome Ku70/Ku80 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA DNA-polymerase 3´ to 5´editing function removes incorrectly inserted nucleotides. Describe Proteinfaktorer som möjliggör detta är Ku70/80 och DNAks som av K Söderlund Leifler · 2009 — associated with cancer due to gain-of-function mutations are called proto- oncogenes binding to Ku70/80, DNA-PKcs is activated by autophosphorylation and.
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Literature references 2005- Immunotechnology

This domain only makes a small contribution to the dimer interface. Ku70/80 is an heterodimeric factor that plays a critical role in the repair of double strand breaks by non-homologous end joining (NHEJ). In this process, Ku is though to facilitate the ligation of broken DNA ends by promoting the recruitment of nuclease, ligases and polymerases necessary for the repair . As DNA end joining would be detrimental to chromosome ends, it was though that Ku has to be kept away from telomeres to prevent deleterious telomere-telomere fusion.

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DNA DAMAGE - DiVA Portal

3A, lane 3), indicating that Ku70/80 and RECQ1 can together bind the fork DNA structure.