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Cho Lec 2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Transfection efficiency of CHO-K1 cells 24 hours post Nucleofection™. CHO-K1 cells (ATCC® CCI-61™) were transfected with program DT-133 and 0.2 μg of pmaxGFP™ Vector in 20 µl Nucleovette™ Strips. 24 hours post Nucleofection™ cells were analyzed on a FACSCalibur™ (Becton Dickinson). Cell viability (% PI-negative cells) is usually around 95% after 24 hours.

.. Both cell lines were cultured in alpha-minimal essential medium (αMEM; Gibco-BRL, New York) supplemented with 10% (vol/vol) fetal calf serum (JRH Biosciences, Kansas), 4 mM l -glutamine, 100 IU of penicillin, 10 μg of streptomycin/ml, nonessential amino acids (Gibco-BRL), and 50 μM β-mercaptoethanol. Cell Lines CHO 5/9 m alpha 3-18 (M-CSF) cells (ATCC #CRL-10154) were obtained from the American Type Culture Collection (ATCC). Cell line CHO-IgG is a proprietary clone expressing a recombinant antibody. Culture media The media used in this study are CHO Animal Component-Free Medium (C5467) and CHO DHFR- Medium (C8862). Cell Culture and Cell The glycosylation mutant cell line, Lec2, derivedfrom CHO Pro-5 cells (,,) was also obtained from the ATCC Example for Nucleofection™ of CHO-K1 cells Transfection efficiency of CHO-K1 cells 24 hours post Nucleofection™.

Other Collection No.: ATCC CCL 61. Amaxa® Cell Line Nucleofector® Kit T. For CHO-K1 [ATCC® CCL-61™, cryopreserved]. Chinese hamster ovary; adherent fibroblastoid cells.

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Chinese hamster ovary, stocked custom serum free media recommended for this cell line include CHO-III-PFM (protein free medium for suspension culture;096-0334-DJ); CHO-III A PFM (protein free medium for adherent culture,097-0147-DK); CD-CHO-A medium (protein free, chemically defined medium for adherent culture Cell Culture and Plasmid Transfection Adherent wild-type CHO-K1 cells (CCL-61, ATCC) and engineered CHO cells were maintained in complete HAMS-F12 (cHAMS-F12) medium (ATCC) containing 10% fetal bovine serum (FBS) (Sigma-Aldrich), 1% HyClone non-essential amino acids (GE Healthcare Life Sciences), and 1% penicillin/streptomycin (P/S) (Gibco by Life Sciences). ..

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This cell line has a chromosome frequency distribution of 50 cells: 2n=22 and its stemline number is hypodiploid. CHO cells are widely used and well-characterized in methods for cell transfection, gene amplification, and clone selection. CHO cells should be cultured in Ham’s F12K (ATCC suggestion) or DMEM modified with 10% FBS. If cells are not doubling every 14-17 hours, supplement the medium with 1-2% FCS. CHO Subculture Protocol (adherent) CHO cells grow quickly and easily and cell doubling time is 14-17 hours. CHO-K1 cells were transiently transfected as illustrated in Figure 1C, with gRNA constitutively expressed by the U6 promoter from plasmid #1 (P1).

24 hours post Nucleofection™ cells were analyzed on a FACSCalibur™ (Becton Dickinson). Cell viability (% PI-negative cells) is usually around 95% after 24 hours. Transfection e ciency The glycosylation mutant cell line, Lec2, derived from CHO Pro-5 cells ( , , ) was also obtained from the ATCC. .. Both cell lines were cultured in alpha-minimal essential medium (αMEM; Gibco-BRL, New York) supplemented with 10% (vol/vol) fetal calf serum (JRH Biosciences, Kansas), 4 mM l -glutamine, 100 IU of penicillin, 10 μg of streptomycin/ml, nonessential amino acids (Gibco-BRL), and 50 μM β … CHO WBL, CHOWBL, CHO-WBL IVGT Chinese Hamster Ovary Genotoxic screening The Chinese hamster ovary CHO cells were originally established as spontaneously transformed cells from an ovarian biopsy by Tijo and Puck (see references below).
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Chinese Hamster Ovary Cell Line, hLT-B: R-hG1 CHO #14 ATCC ® CRL-1 Derived from CHO cells (see Urlaub et al.,1983; Urlaub et al.,1986) Cell line genetically modified. A CHO-K1 DG44 cell line was stably transfected with a two-vector system expressing a model mouse-human chimeric IgG1 antibody. Single cell derived clones were isolated by limiting dilution and expanded. CHO cell line 3-631-436 AM5 ATCC ® PTA-8369™ U.S. Patent Num CHO cells should be cultured in Ham’s F12K (ATCC suggestion) or DMEM modified with 10% FBS. If cells are not doubling every 14-17 hours, supplement the medium with 1-2% FCS. CHO Subculture Protocol (adherent) CHO cells grow quickly and easily and cell doubling time is 14-17 hours. This line is a mutant clone derived from the parental CHO clone Pro-5 (a proline auxotroph, see ATCC CRL-1781) by selection for resistance to wheat germ agglutinin.

Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of recombinant therapeutic proteins. They have found wide use in studies of genetics, toxicity screening, nutrition and gene expression, particularly to express recombinant proteins. Trypsin-nicked wild-type or mutant PA was incubated with CHO-K1 cells at 4°C. The cells were washed twice with PBS and then exposed to buffer at pH 4.8 to induce channel formation.
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av T Karlsson — were shown to localize to the leading edge in migrating CHO cells and astroglial cells, All cells were originally retrieved from ATCC (Teddington, UK). av M Jenmalm — ATCC 55730 i en studie (16) samt. Lactobacillus through regulatory T cells. J Allergy Clin.

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CHO, CHOK1, cho k1 Hamster Chinese ovary A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. ATCC cho e606 cells Cho E606 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Compare CHO/dhFr- from ATCC on Biocompare.com. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists.